MDolors Piulachs Lab
Insect Reproduction Lab
Institut de Biologia Evolutiva (CSIC-UPF)
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Publications
  • Irles, P, Belles, X, Piulachs, MD (2009) Brownie, a Gene Involved in Building Complex Respiratory Devices in Insect Eggshells PLoS ONE [pdf]
  • Revuelta, L, Piulachs, M.D, Bellés, X, Castañera, P, Ortego, F, Díaz-Ruíz, J.R, Hernández-Crespo, P, Tenllado, F. (2009). RNAi of ace1 1 and ace2 in Blattella germanica reveals their differential contribution to acetylcholinesterase activity and sensitivity to insecticides. Insect Biochemistry and Molecular Biology 1-7 [pdf]
  • Irles P, Bellés X, Piulachs, MD. (2009). Identifying genes related to choriogenesis in insect panoistic ovaries by Suppression Subtractive Hybridization. BMC Genomics. 2009 Apr 30;10:206 [pdf]
  • Guidugli-Lazzarini, KR., Mendes do Nascimento, A., Tanaka, E.D., Piulachs, MD, Hartfelder, K., Bitondi, MG and Simões, ZLP (2008). Molecular characterization and expression analysis of candidate vitellogenin and lipophorin receptors in apis mellifera females. Journal of Insect Physiology. 54 [pdf]
  • Ciudad, L.,  Bellés, X., Piulachs, MD (2007). Structural and RNAi characterization of the German cockroach lipophorin receptor, and the evolutionary relationships of lipoprotein receptors. BMC Molecular Biology, 8: 53 [pdf]
  • Martín, D., Piulachs, MD., Cunillera, N., Ferrer, A., Belles, X. (2007). Mitochondrial targeting of farnesyl diphosphate synthase is a widespread phenomenon in eukaryotes, BBA - Molecular Cell Research. 1773, (3): 419-426 [pdf]
  • Pellens, R., D’Haese, C.A., Bellés, X., Piulachs, M-D., Legendre, F., Wheeler, W.C., Grandcolas, P. (2007). The evolutionary transition from subsocial to eusocial behaviour in Dictyoptera: Phylogenetic evidence for modification of the “shift-in-dependent-care” hypothesis with a new subsocial cockroach, Molecular Phylogenetics and Evolution. 43:616-626 [pdf]
  • Treiblmayr K, Pascual N, Piulachs MD, Keller T, Belles X. (2006). Juvenile hormone titer versus juvenile hormone synthesis in female nymphs and adults of the German cockroach, Blattella germanicaJournal of Insect Science. 6:43 [pdf]
  • Ciudad, L., Piulachs, M.D., Bellés, X. (2006). Systemic RNAi of the cockroach vitellogenin receptor results in a phenotype similar to that of the Drosophila yolkless mutant. FEBS Journal. 273: 325-335 [pdf]
  • Guidugli, K.R., Piulachs, M.D., Bellés, X., Lourenço, A.P., Simões, Z.L.P. (2005). Vitellogenin expression in queen ovaries and in larvae of both sexes of Apis mellifera. Archives of Insect Physiology and Biochemistry 59: 211-218 [pdf]
  • Bellés, X., Martín, D., Piulachs, M.D. (2005). The mevalonate pathway and the synthesis of juvenile hormone in insects. Annual Review of Entomology. 50:181-199. [pdf]
  • Murienne, J., Grandcolas, P. Piulachs, M.D., Bellés, X., D'Haese, C., Legendre, F., Pellens, R. and Guilbert, E. 2005. Evolution on a shaky piece of Gondwana: is local endemism recent in New Caledonia? Cladistics 21: 2-7 [pdf]
  • Aguilar, R., Maestro, J.L., Vilaplana, L., Pascual, N., Piulachs, M.D. y Bellés, X. (2003). Allatostatin gene expression in brain and midgut, and activity of synthetic allatostatins on feeding-related processes in the cockroach Blattella germanica. Regulatory Peptides 115: 171-177 [pdf]
  • Cruz, J., Martín, D., Pascual, D., Maestro, J.L., Piulachs, M.D. and  Bellés, X. (2003). A matter of quantity. German cockroach larvae do not make vitellogenin because they do not produce enough juvenile hormone, not because the fat body is incompetent. Insect Biochemistry and Molecular Biology 33(12): 1219-1225 [pdf]
  • Zapata, R., Piulachs, M.D., Bellés, X. (2003). Inhibitors of 3-hydroxy-3-methylglutaryl-CoA reductase decrease fertility in the German cockroach. Correlation between sterilisation in vivo with the inhibition of enzymatic activity in embryo cells. Pest Management Science 59: 1111-1117 [pdf]
  • Piulachs, MD, Guidugli, KR., Barchuk, AR, Cruz, J., Simões, ZLP and Bellés, X. (2003).The vitellogenin of the honey bee, Apis mellifera: structural analysis of the cDNA and expression studies.  Insect Biochemistry and Molecular Biology 33(4):  459-465 [pdf]
  • Zapata R., Piulachs, M.D., Bellés, X. (2002). Ovarian 3-hydroxy-3-methylglutaryl-CoA reductase in Blattella germanica (L.). Pattern of expression and critical role on embryogenesis Journal of Insect Physiology. 48: 675-681 [pdf]
  • Zapata R., Martín, D., Piulachs, M.D., Bellés, X. (2002). Effects of hypocholesterolaemic agents on the expression and activity of 3-hydroxy-3-methylglutaryl-CoA reductase in the fat body of the German cockroach. Archives of Insect Biochemistry and Physiology 49: 177-186 [pdf]
  • Grandcolas, P., Bellés, X., Piulachs, M.D. & D'Haese, C. (2002). Le genre Lauraesilpha Grandcolas, 1997: endémisme, caractères d’appartenance aux Blattidae et séquences d’ARN mitochondrial (Insectes, Dictyoptères, Blattidae, Tryonicinae). Mémoires du Museum National d’Histoire naturelle. 187:117-13 [pdf]
  • Grandcolas, P., Y.C. Park, J.C. Choe, M.D. Piulachs, X. Bellés, C. D'Haese, J.P. Farine & R. Brossut. (2001). What does Cryptocercus kyebangi, n.sp. from Korea reveal about Cryptocercus evolution? A study in morphology, molecular phylogeny and chemistry of tergal glands (Dictyoptera, Blattaria, Polyphagidae). Proc. Acad. nat. Sci. Phila., 151: 61–79. [pdf]
  • Maestro, J.L., Aguilar, R., Pascual, N., Valero, M.-L., Piulachs, M.-D., Andreu, D.,  Navarro, I. and Bellés, X. 2001. Screening of antifeedant activity in brain extracts led to the identification of sulfakinin as a satiety promoter in the German cockroach. Are arthropod sulfakinins homologous to vertebrate gastrins-cholecystokinins? European Journal of Biochemistry, 268 (22):5824-5830 [pdf]
  • Comas, D., Piulachs, M.D., Bellés, X. (2001). Induction of vitellogenin gene transcription in vitro by juvenile hormone in Blattella germanica. Mol. Cell. Endocrinol. 183(1-2): 93-100 [pdf]
  • Martín, D., Piulachs, M.D. and Raikhel, A.S. (2001). "A novel GATA factor transcriptionally represses yolk protein precursor genes in the mosquito Aedes aegypti via interaction with the CtBP co-repressor". Molecular and Cellular Biology. 21: 164-174 [pdf]
  • Belles X, Piulachs MD, Pascual N, Maestro, JL, Martin, D. (2000). On the role of Juvenile Hormone in vitellogenesis in cockroaches.  Physiological entomology 25 (3): 207-208 [pdf]
  • Comas, D. Piulachs, M.D., Bellés, X. (2000). Vitellogenin of Blattella germanica (l.) (Dictyoptera, Blattellidae): Nucleotide sequence of the cDNA and analysis of the protein primary structure. Arch. Insect Biochem Physiol. 45: 1-11 [pdf]
  • Peralta, E., Vilaplana, Ll., Pascual, N., Carreño, C., Piulachs, M.D., Andreu, D., Bellés, X. (2000). A microdialysis study of allatostatin degradation in Blattella germanica (L.) (Dictyoptera, Blattellidae). Physiol. Entomol. 25: 254-259 [pdf]
  • Bellés, X., Piulachs, M.D., Pascual, N., Maestro, J.L., Martín, D., Vilaplana, Ll. (1999). Regulation of vitellogenesis in the German cockroaches and the problem of cyclicity. In: Recent Developments in Comparative Endocrinology and Neurobiology. Pp 223-227 (Roubos, Wendelaar Bonga, Vaudry and De Loof, eds.)
  • Comas, D., Piulachs, M.D., Bellés, X. (1999). Fast induction of vitellogenin gene expression  by juvenile hormone III in the cockroach Blattella germanica (L.) (Dictyoptera, Blattellidae). Insect Biochemistry and Molecular Biology. 29: 821-827.[pdf]
  • Vilaplana, Ll., Maestro, J.L., Piulachs, M.D., Bellés, X. (1999). Modulation of cardiac rhythm by allatostatins in the cockroach Blattella germanica (L.)(Dyctioptera, Blattellidae). Journal of insect Physiol. 45. 1057--1064.[pdf]
  • Vilaplana, Ll., Maestro, J.L., Piulachs, M.D., Bellés, X. (1999). Determination of allatostatin levels in relation to the gonadotropic cycle in the female of Blattella germanica. (L.)(Dyctioptera, Blattellidae). Physiol. Entomology 24: 213-219. [pdf]
  • Martin, D., Piulachs, M.D. & Bellés, X. (1998). Allatostatin inhibits vitellogenin release in a cockroach. Annals of the New York Academy of Sciences. 839:241-242.
  • Martín, D., Piulachs, M.D., Comas, D., Bellés, X. (1998). Isolation and sequence of a partial vitellogenin cDNA from the cockroach Blattella germanica (L.) (Dictyoptera, Blattellidae), and characterization of the vitellogenin gene expression. Archives of Insect Biochemistry and Physiology 38: 137-146. [pdf]
  • Maestro, J.L., Bellés, X., Piulachs, M.D., Thorpe, A. & Duve, H. (1998). Localization of allatostatin-immunoreactive material in the central nervous system, stomatogastric nervous system, and gut of the cockroach Blattella germanica. Archives of Insect Biochemistry and Physiology 37: 269-282. [pdf]
  • Osorio, S., Piulachs, M.D. & Bellés, X. (1998). Feeding, corpora allata development and allatostatins in the cockroach Blattella germanica (L.) (Dictyoptera, Blattellidae). Journal of Insect Physiology 44:31-38. [pdf]
  • Piulachs, M.D., Vilaplana, Ll., Bartolomé, J.M., Carreño, C.,  Martín, D, González-Muñiz, R., Herranz, R., García-López, M.T., Andreu, D. & Bellés, X. (1997). Ketomethylene and methyleneamino pseudopeptide analogues of insect allatostatins inhibit juvenile hormone and vitellogenin production in the cockroach Blattella germanica. Insect Biochemistry and Molecular Biology. 27(10): 851-858. [pdf]
  • Bellés, X. & Piulachs, M.D. (1997). Alatostatinas. Una familia singular de neuropéptidos de insectos. En: Péptidos; Química, Biología y Biomedicina (D. Andreu & L. Rivas, eds.). Colección Nuevas tendencias, CSIC, Madrid.
  • Casals, N., Martín, D., Buesa, C., Piulachs, M.D., Hegardt, F.G.& Bellés, X. (1997). Expression and activity of 3-hydroxy-3-methylglutaryl-CoA synthase and reductase in the fat body of ovariectomized and allatectomized Blattella germanica. Physiological Entomology 22: 6-12. [pdf]
  • Martín, D., Piulachs, M.D. & Bellés, X. 1996. Inhibition of vitellogenin production by allatostatin in the German cockroach. Molecular and Cellular Endocrinology 121: 191-196. [pdf]
  • Casals, N., Buesa, C., Piulachs, M.D., Cabañó, J., Marrero, P.F., Bellés, X.& Hegardt, F.G. (1996). Coordinated expression and activity of 3-Hydroxy-3-methylglutaryl Coenzime A synthase and reductase in the fat body of Blattella germanica (L.) during vitellogenesis. Insect Biochemistry and Molecular Biology 26: 837-843. [pdf]
  • Vilaplana, Ll., Piulachs, M.D.& Bellés, X. (1996). The conglobate gland of Blattella germanica (L.) (Dictyoptera, Blattellidae). Maturation, juvenile hormone dependency and changes during spermatophore formation. Invertebrate Reproduction and Development, 29: 167-172.
  • Vilaplana, Ll., Piulachs, M.D. y Bellés, X. (1996). “Inhibition of juvenile hormone during the formation of the spermatophore in Blattella germanica (L.) (Dictyoptera, Blattellidae)” Arch. Insect Biochem. Physiol. 32: 559-566. [pdf]
  • Martín, D., Piulachs, M.D., Bellés, X. (1996). "Production and extraovarian processing of vitellogenin in ovariectomized Blattella germanica (L.) (Dictyoptera, Blattellidae). J. Insect Physiol. 42: 101-105. [pdf]
  • Martín, D., Piulachs, M.D., Bellés, X. (1995). "Production of vitellogenin in vitro by the  periovaric fat body of Blattella germanica (L.) (Dictyoptera, Blattellidae). Inv. Rep. Develop. 28: 171-176.
  • Martin, D., Piulachs, M.D., Bellés, X. (1995). "Patterns of haemolymph vitellogenin and ovarian vitellin in the german cockroach, and the role of juvenile hormone". Physiol. entomol. 20: 59-65.
  • Maestro, J.L., Danés, M.D., Piulachs, M.D., Cassier, P., Bellés, X. (1994). "Functionality and ultrastructure of corpora allata in ovariectomized Blattella germanica (L.) (Dictyoptera, Blattellidae)". Physiol. entomol. 19: 342-348.
  • Buesa, C., Martínez-González, J., Casals, N., Haro, D., Piulachs, M.D., Bellés, X., G.-Hegardt. F. (1994). "Blattella germanica has two HMG-CoA synthase genes. Both are regulated in the ovary during gonadotrophic cycle". J. Biol. Chem. 269: 11707-11713. [pdf]
  • M.D. Piulachs. (1994). Las cucarachas. Ciclo biológico y estrategias reproductivas. Parasitis 3: 22-25.
  • Bellés, X., Maestro, J.L., Piulachs, M.D., Johnsen, A., Duve, H., Thorpe, A. (1994)."Allatostatic neuropeptides from the cockroach Blattella germanica (L.) (Dictyoptera, Blattellidae). Identification, immunolocalization and activity" Reg. Peptides. 53: 237-247. [pdf]
  • Martínez-González, J., Buesa, C., Piulachs, M.D., Bellés, X., Hegardt, F.G. (1993). "3-Hydroxy-3-methylglutaryl-coenzyme-A synthase from Blattella germanica. Cloning, expression, developmental pattern and tissue expression". Eur. J. Biochem. 217: 691-699. [pdf]
  • X. Bellés, P. Cassier, X. Cerdá, N. Pascual, M. André, Y.Rosso, M.D. Piulachs (1993). Induction of choriogenesis by 20-hydroxyecdysone in the german cockroach. Tissue and Cell. 25 (2): 195-204. [pdf]
  • J. Martínez-Gonzalez, C. Buesa, M.D. Piulachs, X. Bellés, F.G. Hegardt (1993). Molecular cloning, developmental pattern and tissue expression of 3-hydroxy-3-methylglutaryl coenzime A reductase of the cockroach Blattella germanica. Eur. J. Biochem. 213 (1):233-241. [pdf]
  • J.L. Maestro, M.D. Piulachs, X. Bellés (1993). Autoinhibition of juvenile hormone production. The case of the cockroach Blattella germanica (L.) Experientia. 49: 320-323.
  • P. Cassier, M. André, D. Pastor, M.D. Piulachs, X. Bellés (1993). Age-dependent neurosecretion release induced by dopamine in the corpora cardiaca of Blattella germanica (L.) (Dictyoptera: Blattellidae). Int. J. Insect Morphol. & Embryol. 22(1): 1-11. [pdf]
  • García-Alonso, M., Piulachs, M.D, X. Bellés (1992). Influencia de los corpora allata en el desarrollo de las glándulas sexuales accessorias del macho de Blatta orientalis. Rev. Esp. Fisiol. 48:77-82.
  • Bellés y M.D. Piulachs (1992). Juvenile hormone and maturation of male accessory reproductive glands in the cockroach Blattella germanica (L.). En: Recent Progress in Insect Juvenile Hormone Research: Fundamental and applied approach. (B. Mauchamp, F. Couillaud, J.C. Baehr Eds.)pp. 101-109. Paris, Francia.
  • M.D. Piulachs, F. Couillaud (1992). Differential Stimulation of juvenile hormone synthesis  induced by mevalonate and mevalonolactone in Blattella germanica (L.). J. Insect Physiol. 38: 555-560. [pdf]
  • A.G. González, I.A. Jimenez, A.G. Ravelo, X. Bellés, M.D. Piulachs (1992). Antifeedant activity of Dihydro-ß-agarofuran sesquiterpenes from celastraceae against Spodoptera littoralis. Biochem. System. Ecol. 20: 311-315. [pdf]
  • M.D. Piulachs, J.L. Maestro, X. Bellés (1992). Juvenile hormone production and accessory reproductive gland development during sexual maturation of male Blattella germanica (L.) (Dictyoptera, Blattellidae). Comp. Biochem. Physiol. 102A: 477-480. [pdf]
  • N. Pascual, X. Cerdá, B. Benito, J. Tomás, M.D. Piulachs, X. Bellés. X. (1992). Ovarian ecdysteroid levels and basal oocyte development during maturation in the cockroach Blattella germanica (L.). J. Insect Physiol. 38: 339-348. [pdf]
  • D. Pastor, M.D. Piulachs, P. Cassier, M. André, X. Bellés. (1991). Étude in vivo et in vitro de l'action de la dopamine sur la croissance des ovocytes et la production d'hormone juvénile chez Blattella germanica (L.) (Dictyoptera; Blattellidae). C. R. Acad. Sci. III Paris 313:  207-212.
  • M. Tamarelle, X. Bellés, M.D. Piulachs y J. Girardie.(1990). Gastrin-cholecystokinin-like immunoreactivities in the brain and retrocerebral neuroendocrine complex of the cockroach Blattella germanica. Histochemistry 93, 433-438. [pdf]
  • M.D. Piulachs, X. Bellés. (1989). Influencia de la cópula en la duración del primer ciclo gonotrófico y en la formación de la ooteca de Blattella germanica (L.) (Dictyoptera, Blattellidae). Boletin de Sanidad Vegetal-Plagas 14 (3), 357-362.
  • X. Bellés, M.D. Piulachs. (1989). Stimulatory modulation of corpora allata activity in adult females of the cockroach Blattella germanica. Gen. comp. Endocrinol. 74 (2), 305.
  • M.D. Piulachs, X. Bellés.(1989). Stimulatory activity of cysteamine on juvenile hormone release in adult females of the cockroach Blattella germanica. Comp. Biochem. Physiol. 94A (4), 795-798. [pdf]
  • X. Bellés, F. Camps, J. Casas, B. Mauchamp, M.D. Piulachs y A. Messeguer. (1989). Stimulating action of methyl 12,12,12-trifluorofarnesoate on the in vitro juvenile hormone III biosynthesis in Blattella germanica. Arch. Insect Biochem. Physiol. 11, 257-270. [pdf]
  • M.D. Piulachs, P. Cassier, X. Bellés. (1989). Ultrastructural changes induced by precocene II and 3,4-dihydroprecocene II on the corpora allata of Blattella germanica: an in vivo and in vitro study. Cell Tissue Res. 258, 91-99. [pdf]
  • X. Bellés y M.D. Piulachs. (1989). Ocurrence of brain allatostatic factors in the cockroach  Blattella germanica. Acta Entomol. Bohemos. 86, 161-166.
  • M.D. Piulachs. (1988). Neurosecrecion en insectos. En: Insecticidas biorracionales (Bellés, X., Ed.). pp: 227-247. C.S.I.C. (Colección Nuevas Tendencias) Madrid.
  • M.D. Piulachs. (1988). Acción gonadotrófica de la hormona juvenil en  hembras de Blattella germanica alatectomizadas o sometidas a ayuno prolongado. Miscel.lània Zoologica 12(1988):121-124. [pdf]
  • X. Bellés y M.D. Piulachs (1988)."Effects of somatostatin on in vitro juvenile hormone III release in Blattella germanica (L.) (Dictyoptera, Blattellidae)" En: Endocrinological Frontiers in Physiological Insect Ecology (Zabza, A., Sehnal, F., Denlinger, D.L., eds)pp. 875-879. Tech. Univ. Wroclaw Poland.
  • X. Bellés, M. Fabriàs y M.D. Piulachs (1988)."Antifeedent effects of precocenes on Spodotera littoralis (Boisduval)(Lepidoptera, Noctuidae)". En: Endocrinological Frontiers in Physiological Insect Ecology (Zabza, A., Sehnal, F.C Denlinger, D.L., eds.)pp. 75-79. Tech. Univ. Wroclaw Poland.
  • X. Bellés, F. Camps, J. Casas, J. Lloria, A. Messeguer, M.D. Piulachs y F.J. Sánchez. (1988). In vivo and in vitro effects of compactin in liposome carriers on juvenile hormone biosynthesis in adult females of Blattella germanica. Pestic. Biochem. Physiol. 32, 1-10.
  • X. Bellés y M.D. Piulachs. (1988). Modulation by somatostatin of juvenile hormone release in a cockroach. Naturwissenschaften 75, 413-145.
  • X. Bellés, F. Camps, J. Casas, A. Messeguer, M.D. Piulachs. (1988). In vitro inhibition of JH III biosynthesis by precocene II on Blattella germanica. J. Insect Physiol. 34 (6), 457-461. [pdf]
  • X. Bellés, J. Casas, A. Messeguer, M.D. Piulachs. (1987). In vitro biosynthesis of JH III by the corpora allata of Blattella germanica adult females. Insect Biochem. 17 (7), 1007-1010. [pdf]
  • J. Casas, J. Roselló, E. Gelpí, F. Camps, M.I. Baldellou, X. Bellés, A. Messeguer, M.D. Piulachs. (1986). Determination by HPLC-RIA of inmunoreactive prostaglandin E2  in Blattella germanica and Gryllus bimaculatus. Rev. Esp. Fisiología 42 (4), 507-512.
  • J. Casas, J. Roselló, E. Gelpí, F. Camps, M.I. Baldellou, X. Bellés, A. Messeguer, M.D. Piulachs. (1985). Determinación de prostaglandina E2 en adultos de Blattella germanica (L.) (Dictyoptera, Blattellidae) y de Gryllus bimaculatus de Geer (Orthoptera, Gryllidae). Bolm. Soc. port. Ent. (suppl.1) 3, 429-437.
  • X. Bellés, A. Messeguer, M.D. Piulachs. (1985). Efectos antagonistas de la hormona juvenil inducidos por los precocenos sobre hembras de Blattella germanica (L.) (Dictyoptera, Blattellidae). Bolm. Soc. port. Ent. (Suppl. 1) 3, 393-402.
  • M.D. Piulachs y X. Bellés. (1985). Efectes dels precocens sobre els corpora allata de Blattella germanica (L.) (Dictyoptera, Blattellidae). Butll. Soc. Cat. Biol. 38, 39-44.
  • X. Bellés, F. Camps, J. Coll, M.D. Piulachs. (1985). Insect antifeedant activity of clerodane diterpenoids against larvae of Spodoptera littoralis (Boisd.) (Lepidoptera). J. Chem. Ecol. 11 (10), 1439-1445.
  • X. Bellés, A. Messeguer, M.D. Piulachs. (1985). Sterilization induced by precocenes on females of Blattella germanica (L.): short- and long-term effects. Z. Ang. Ent. 100, 409-417.
  • X. Bellés, M.I. Baldellou y M.D. Piulachs (1984). "Actividad ovicida del precoceno 2 sobre la galeruca del aliso Agelastica alni (Col. Chrysomelidae)". Bol. Est. Central Ecol. 13 (25): 97-99.
  • X. Bellés y M.D. Piulachs. (1983). Desarrollo de los corpora allata, oocitos y glándulas colaterales durante el primer ciclo gonotrófico de Blattella germanica. Rev. Esp. Fisiología 39, 149-154.